Cell Line Lysates
Cayman Chemical Humn GlucocortIcoId RECPTR 384
Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.[INDIGO Catalog Nos. IB00201-32, IB00201, IB00202]
Cayman Chemical KIgamIcIn C 2.5mg
An antitumor antibiotic; active against various strains of S. aureus, including MRSA, as well as M. luteus and B. subtilis (MICs = 0.05-0.2 UG/ml for all); is cytotoxic to PANC-1 cells in nutrient-deprived media
Cayman Chemical OlIgomycIn D 2.5mg
A macrolide antibiotic that inhibits the mitochondrial F1FOATPase; inhibits K-Ras plasma membrane localization in MDCK cells with an IC50 value of 3.49 nM and is cyctoxic to SW620 colon cancer cells with an IC50 value of 36 µM
Sino Biological Recombinant Human CNR1 Protein (VLP, Flag-Strep & His tag)
A DNA sequence encoding the Human CNR1 (P21554-1) (Met1-Ser414) was expressed, with a Flag tag followed by a Strep Tag at the N-terminus, a polyhistidine tag at the C-terminus. This protein was prepared using our VLP platform.As an innovative technology platform, VLPs are generated through the self-assembly of viral proteins in HEK293 cells. This process efficiently integrates membrane proteins onto the VLP surface, preserving their native conformation and yielding soluble, high-concentration proteins that are ideally suited for immunization and antibody screening.
Sino Biological Human MUC-1 Protein (aa890-1158, His Tag), Biotinylated
A DNA sequence encoding the Human MUC1 (P15941-1) (Ser890-Gly1158) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.
Sino Biological Recombinant Human LGR5 Protein (VLP, Flag & His-Strep Tag)
A DNA sequence encoding the Human LGR5 -VLP (O75473) (Gly22-Ser844) was expressed, with a Flag tag at the N-terminus, a polyhistidine tag and a strep tag at the C-terminus.
Cell Biolabs Inc 293AAV Cell Line for AAV Packaging
Cell Biolabs' 293AAV Cell Line is a permanent line established from primary embryonic human kidney transformed with human adenovirus type 5 DNA. The genes encoded by the E1 region of adenovirus (E1a and E1b) are expressed in these cells and participate in transactivation of viral promoters, allowing these cells to produce high levels of protein. The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production. It offers several advantages over the regular 293 cells: (1) Larger cell surface area resulting higher transfection and better yield of AAV; (2) Flattened morphology; and (3) Firm attachment to culture plates, ideal for large scale culture and AAV production.
Cayman Chemical Humn LIver X ReceptrAlpha 96
LXR Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments. [INDIGO Catalog Nos. IB00311-32, IB00311, IB00312]
Sino Biological Recombinant Human Desmoglein-2 Protein (His Tag), HPLC-verified
A DNA sequence encoding the Human DSG2 (Q14126) (Ala50-Gly609) was expressed with a polyhistidine tag at the C-terminus.
Cayman Chemical ThIelavIn B 2.5mg
A fungal metabolite that inhibits cyclooxygenase, blocking both the conversion of AA to PGH2 and the conversion of PGH2 to PGE2 (IC50s = 40 and 9 µM, respectively); also inhibits the reverse transcriptase of avian myeloblastosis virus, bacterial transglycosylases, and telomerase activity
Rockland Immunochemicals PC-3 Whole Cell Lysate 500µg
Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 10-20 µL depending on the size format of your gel.
Sino Biological Recombinant Human CD98 Protein (FITC conjugated, His Tag)
A DNA sequence encoding the Human SLC3A2 (P08195) (Arg206-Ala630) was expressed, with a polyhistidine tag at the N-terminus.
Cayman Chemical AmIdepsIn D 2.5mg
A fungal metabolite that inhibits DGAT activity, preventing triacylglycerol formation (IC50s = 17.5 and 2.8 µM in rat liver microsomes and Raji cells, respectively)
